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sandwich enzyme-linked immunosorbent assay [1 record]
Record 1 - internal organization data 2006-03-16
Record 1, English
Record 1, Subject field(s)
- Immunology
Record 1, Main entry term, English
- sandwich enzyme-linked immunosorbent assay 1, record 1, English, sandwich%20enzyme%2Dlinked%20immunosorbent%20assay
Record 1, Abbreviations, English
- SELISA 1, record 1, English, SELISA
Record 1, Synonyms, English
- sandwich ELISA 1, record 1, English, sandwich%20ELISA
Record 1, Textual support, English
Record number: 1, Textual support number: 1 CONT
We have generally found that irradiated merrotitre plates have a greater capacity than non-irradiated plates to bind allergen or whole cells. Once bound, the allergen can become detached from the plate and this is particularly critical if it occurs during the assay ... One solution to this problem is to link the antigen to the plate via an antibody in a sandwich ELISA (SELISA). Polyclonal rabbit and mouse monoclonal antibodies have been used to bind allergens to microtiter plates. 1, record 1, English, - sandwich%20enzyme%2Dlinked%20immunosorbent%20assay
Record 1, French
Record 1, Domaine(s)
- Immunologie
Record 1, Main entry term, French
- technique immunoenzymatique en sandwich
1, record 1, French, technique%20immunoenzymatique%20en%20sandwich
feminine noun
Record 1, Abbreviations, French
Record 1, Synonyms, French
- méthode immuno-enzymatique en sandwich 1, record 1, French, m%C3%A9thode%20immuno%2Denzymatique%20en%20sandwich
feminine noun
- technique du sandwich 2, record 1, French, technique%20du%20sandwich
feminine noun
Record 1, Textual support, French
Record number: 1, Textual support number: 1 CONT
Techniques ELISA [...] On parle de techniques immunoenzymatiques directes, indirectes, en sandwich, avec ou sans compétition [...] la méthode en sandwich (comporte les) étapes suivantes. Les anticorps spécifiques sont fixés passivement sur le support solide. Puis on dépose l'échantillon testé; l'antigène présent se fixera sur l'anticorps. On applique alors l'anticorps spécifique, éventuellement lié à une enzyme, suivi de l'anticorps anti-immunoglobuline spécifique de l'espèce où est produit l'anticorps [...] On ajoute enfin le substrat de l'enzyme. 1, record 1, French, - technique%20immunoenzymatique%20en%20sandwich
Record number: 1, Textual support number: 1 OBS
Pluriel : des sandwichs; des sandwiches. 3, record 1, French, - technique%20immunoenzymatique%20en%20sandwich
Record number: 1, Textual support number: 2 OBS
sandwichs (pl.) : Cette graphie, puisée des Rectifications de l'orthographe recommandées par le Conseil supérieur de la langue française, est attestée dans le Petit Robert (2006). 4, record 1, French, - technique%20immunoenzymatique%20en%20sandwich
Record 1, Spanish
Record 1, Textual support, Spanish
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